Current Advances in Nanomaterial-associated Micro and Nano-devices for SARS-CoV-2 Detection

COVID-19 spread rapidly around the world in 18 months, with various forms of variants caused by severe acute respiratory syndrome (SARS-CoV). This has put pressure on the world community and created an urgent need for understanding its early occurrence through rapid, simple, cheap, and yet highly accurate diagnosis. The most widely adopted method as of today is the real-time reverse-transcriptase polymerase chain reaction. This test has shown the potential for rapid testing, but unfortunately, the test is not rapid and, in some cases, displays false negatives or false positives. The nanomaterials play an important role in creating highly sensitive systems, and have been thought to significantly improve the performance of the SARSCoV- 2 protocols. Several biosensors based on micro-and nano-sensors for SARS-CoV-2 detection have been reported, and they employ multi-dimensional hybrids on sensing surfaces with devices having different sizes and geometries. Zero-to-three-dimension nanomaterial hybrids on sensing surfaces, including nanofilm hybrids for SARS-CoV-2 detection, were employed with unprecedented sensitivity and accuracy. Furthermore, the sensors were nanofluidic and mediated high-performance SARS-CoV-2 detection. This breakthrough has brought the possibility of making a biosystem on a chip (Bio-SoC) for rapid, cheap, and point-of-care detection. This review summarises various advancements in nanomaterial-associated nanodevices and metasurface devices for detecting SARS-CoV-2.

Customized Luminescent Multiplexed Quick-Response Codes as Reliable Temperature Mobile Optical Sensors for eHealth and Internet of Things

The need to sense and track in real time through sustainable and multifunctional labels is exacerbated by the COVID-19 pandemic, where the simultaneous measurement of body temperature and the fast tracking of people is required. One of the big challenges is to develop effective low-cost systems that can promote healthcare provision everywhere and for that, smarter and personalized Internet of things (IoT) devices are a pathway in large exploration, toward cost reduction and sustainability. Using the concept of color-multiplexed quick response (QR) codes, customized smart labels formed by two independent layers and smart location patterns provide simultaneous tracking and multiple synchronous temperature reading with maximum sensitivity values of 8.5% K-1 in the physiological temperature range, overwhelming the state-of-the-art optical sensor for healthcare services provided electronically via the internet (eHealth) and mobile sensors (mHealth).

The SMeFT Decks: A Card-Based Ideation Tool for Designing Hybrid Digital Boardgames for Distanced Play

Increasing numbers of people have turned to playing boardgames with physically distant friends and family via technological tools, particularly since the start of the COVID-19 pandemic. However, commercial hybrid digital boardgames (HDBs) are designed for co-located, rather than distanced, play and there is a need for more specific tools to support hybrid game design. This paper introduces the SMeFT Decks, a set of card decks to aid in the design of HDBs for distanced play, which support Story, Mechanism, Function and Technology. We describe the use of these cards for design ideation and demonstrate four game concepts for distanced play stemming from the use of these decks in participatory workshops. We report evaluative feedback from a pilot study and from 46 participants who used these cards across nine design workshops and reflect on what we learned from observing this process. Results suggest that the SMeFT Decks are a productive ideation tool for aiding in the design of HDBs for distanced play in collaborative workshop settings. © 2022 ACM.

Reciprocity and design for an era of compressed temporal and spatial scale

Haraway and others have suggested reciprocity with the non-human world is a pathway to un-derstanding our humanness. Two urgent trends accelerate our need for this reciprocity: the first is the COVID-19 pandemic as a harbinger of future pandemics, and the second is our changing planetary climate. Our present time is increasingly becoming a "present-future,” linked irreversibly by scientific models to specific future states of our planet and local regions. At the same time our bodies are co-evolving with a virus in a global reciprocal process with no end in sight, collapsing our sense of scale and separation among bodies. A long view of time in the past could act as a counterbalance to this experience. Bringing the longue durée model of time into our present requires reestablishing our knowledge of a long-term past in which humans adapted to major changes in climate earlier in the Holocene. Forms of future urban adaptation can embody reciprocity by emphasizing strategies that anticipate change rather than seeking to prevent it, leap-ing forward in time to embrace global changes we are no longer able to prevent. © 2022 Author(s).

Cultivating Connections with Farm to School Activities During School Closures

To engage youth and their families in a web-based hybrid club for farm to school activities as an alternative to school-based activities when schools closed due to the pandemic.Farm to school programs increase children's access to and knowledge of fresh and local foods primarily through experiential learning (Kolb's Experiential Learning Theory) such as gardening, tasting food and field trips.Youth, grades 2-6, and their families who experienced disruption in usual learning and social activities due to COVID-19 restrictions.Experiential activities are the hallmark of farm to school education. In March 2020 all hands-on, school based activities planned by Extension faculty were cancelled due to the pandemic. Faculty responded by offering garden-cooking clubs using a web-based hybrid approach independent of schools. Faculty facilitated bi-weekly virtual meetings of 30 minutes for 6-8 weeks for youth and their families. Asynchronous activities were also offered via a website, kits, video demonstrations, farmers market vouchers and when possible, farm tours.Adult caregivers received a survey at the end of the 6-8 week club sessions to evaluate their child's and family's level of engagement including queries on how much time they spent using web-based tools and how likely they were to start a garden.One hundred and ninety youth, grades 2-6, and their families participated in 4 virtual and/or hybrid clubs delivered by Extension faculty from March 2020 to October 2020. Caregiver responses from the survey (n = 46) indicated 83% of families expanded or started a garden. On average youth and/or families spent 47 minutes per week engaged in program activities including online learning.It appears that virtual programming can actively engage youth and their families in farm to school education and may be a tool to supplement resource intensive hands-on strategies in the future. Further research is need to determine which components are best suited for web-based delivery vs in person delivery.

Synthesis of [60]Fullerene Hybrids Endowed with Steroids and Monosaccharides: Theoretical Underpinning as Promising anti-SARS-CoV-2 Agents.

Cyclopropanation reactions between C60 and different malonates decorated with monosaccharides and steroids using the Bingel-Hirsch methodology have allowed the obtention of a new family of hybrid compounds in good yields. A complete set of instrumental techniques has allowed us to fully characterize the hybrid derivatives and to determine the chemical structure of monocycloadducts. Besides, the proposed structures were investigated by cyclic voltammetry, which evidenced the exclusive reductive pattern of fullerene Bingel-type monoadducts. Theoretical calculations at the DFT-D3(BJ)/PBE 6-311G(d,p) level of the synthesized conjugates predict the most stable conformation and determine the factors that control the hybrid molecules' geometry. Some parameters such as polarity, lipophilicity, polar surface area, hydrophilicity index, and solvent-accessible surface area were also estimated, predicting its potential permeability and capability as cell membrane penetrators. Additionally, a molecular docking simulation has been carried out using the main protease of SARS-CoV-2 (Mpro) as the receptor, thus paving the way to study the potential application of these hybrids in biomedicine.

Light‐Driven Catalyst‐Free Access to Phthalazines: Entry to Antiviral Model Drugs by Merging Domino Reactions**

We report the development of a metal-free four-step one-pot synthetic strategy to access high-value functionalized phthalazines using o-methyl benzophenones as starting compounds. Combining a light-mediated enolization of o-methyl benzophenones/Diels-Alder reaction domino process with a subsequent deprotection/aromatization domino reaction in one-pot leads to sustainable and efficient organic synthesis. The tangible advantages, i. e., absence of catalysts or additives, utilization of commercially available and/or easily accessible substrates, mild reaction conditions, simplicity, and single work-up procedure, make this combined process highly appealing for the direct construction of various 1-aryl-phthalazines. Importantly, in vitro bioactivity evaluation of these newly prepared heterocyclic compounds demonstrated a strong antiviral efficacy against major human pathogens like HCMV and SARS-CoV-2.

Overview of Solar–Wind Hybrid Products: Prominent Challenges and Possible Solutions

Solar and wind power systems have been prime solutions to the challenges centered on reliable power supply, sustainability, and energy costs for several years. However, there are still various challenges in these renewable industries, especially regarding limited peak periods. Solar–wind hybrid technology introduced to mitigate these setbacks has significant drawbacks and suffers from low adoption rates in many geographies. Hence, it is essential to investigate the challenges faced with these technologies and analyze the viable solutions proposed. This work examined solar–wind hybrid plants’ economic and technical opportunities and challenges. In the present work, the pressing challenges solar–wind hybrids face were detailed through extensive case studies, the case study of enabling policies in India, and overproduction in Germany. Presently, the principal challenges of solar–wind hybrids are overproduction, enabling policies, and electricity storage. This review highlights specific, viable, proposed solutions to these problems. As already recorded in the literature, it was discovered that academic research in this space focuses majorly on the techno-economic and seemingly theoretical aspects of these hybrid systems. In contrast, reports and publications from original equipment manufacturers (OEMs) and engineering, procurement, and construction engineers (EPCs) are more rounded, featuring real-life application and implementation.

Don’t lose focus on diseases of poverty in COVID-19 crisis

Scientists in Africa, Asia and Europe from a range of disciplines have been researching these diseases of poverty as part of Zoonoses and Emerging Livestock Systems (ZELS), a United Kingdom Department for International Development (DFID) and UK Research and Innovation funded programme. Managing zoonoses A finding that treating cattle with insecticides to combat ticks and tsetse flies can contain the risk of sleeping sickness in people offers the possibility of simple and cost-effective disease-management strategies. Discovering that the majority of schistosomiasis transmission and sickness in Senegal and Niger is driven not by a human schistosome species, as had been previously assumed, but through schistosome species from people and their livestock combining to form highly transmissible viable parasitic hybrids, has helped ensure the WHO embraces a One Health approach to eliminating of a disease that infects more than 240 million people globally.

Charge transport properties of SARS-CoV-2 Delta variant (B.1.617.2).

The B.1.617.2 (Delta) variant of concern is causing a new wave of infections in many countries. In order to better understand the changes of the SARS-CoV-2 mutation at the genetic level, we selected six mutations in the S region of the Delta variant compared with the native SARS-CoV-2 and get the conductance information of these six short RNA oligonucleotides groups by construct RNA: DNA hybrids. The electronic characteristics are investigated by the combination of density functional theory and non-equilibrium Green's function formulation with decoherence. We found that conductance is very sensitive to small changes in virus sequence. Among the 6 mutations in the Delta S region, D950N shows the largest change in relative conductance, reaching a surprising 4104.75%. These results provide new insights into the Delta variant from the perspective of its electrical properties. This may be a new method to distinguish virus variation and possess great research prospects.

Advances in Synthetic Strategies and Medicinal Importance of Benzofurans: A Review

Out of the many heterocycles that exhibit pharmaceutical and therapeutic properties, benzofurans remain the most eye-catching to scientists ever since their discovery. From being present in natural products to synthetic analogs they show diverse biological properties which are used to treat various diseases. Interestingly, some of the benzofuran hybrids were docked against COVID-19 Main Protease and it showed appealing results. Apart from medicinal properties it also exhibits some non-drug applications. The structure activity relationship of benzofuran derivatives in many infections draws attention to the extent where drugs can be produced in a short time span which highlights their significance in medicinal chemistry. There are considerable reaction schemes to synthesize this moiety either simple one pot reactions or multistep. Here, we emphasize on the chemistry of benzofurans, synthesis and its derivatives in the recent years, some of which show notable anti-tumor, anti-fungal, anti-mycobacterial and anti-oxidant activities.

Hybrid Nanocomposites of Plasmonic Metal Nanostructures and 2D Nanomaterials for Improved Colorimetric Detection

Plasmonic phenomena and materials have been extensively investigated for a long time and gained popularity in the last few years, finding in the design of the biosensors platforms promising applications offering devices with excellent performances. Hybrid systems composed of graphene, or other 2D materials, and plasmonic metal nanostructures present extraordinary optical properties originated from the synergic connection between plasmonic optical effects and the unusual physicochemical properties of 2D materials, thus improving their application in a broad range of fields. In this work, firstly, an overview of the structures and properties of 2D nanomaterials will be provided along with the physics of surface plasmon resonance and localized surface plasmon resonance. In the second part of the work, some examples of colorimetric biosensors exploiting the outstanding properties of hybrids nanocomposites will be presented. Finally, concluding perspectives on the actual status, challenges, and future directions in plasmonic sensing biosensing will be provided. Special emphasis will be given to how this technology can be used to support digitalization and virtualization in pandemic handling.

Hybrid university didactics: creative digital identity and multimodality

The incipient interest in hybrid and collaborative didactics in times of COVID-19 highlights the fundamental role of digital hybrids in the training of university students. New mobile devices allow the ubiquity of teaching-learning processes. But they not only allow the possibility of sharing and communicating at any time and place. In these devices, different tools that integrate different representation systems converge, and offer new forms of construction, representation and communication of knowledge. From the questioning of the hegemonic technological culture, and from a didactic approach that advocates comprehensive and critical teaching, we want to present the creative functions of mobile devices so that students can build their own texts, new multimodal narratives, for the construction of a creative digital identity. An identity that allows them to perceive, analyse, reflect, produce, communicate and participate in their environment in a collaborative way. We present a transgressive didactic proposal based on photographic narration that displays new semiotic ways of building knowledge and making it known. We discuss its value to rethink other functions of academic knowledge and assessment, beyond achievement tests, critical dimensions in the University.

Crystallographic study, biological assessment and POM/Docking studies of pyrazoles-sulfonamide hybrids (PSH): Identification of a combined Antibacterial/Antiviral pharmacophore sites leading to in-silico screening the anti-Covid-19 activity.

The discovery and development of new potent antimicrobial and antioxidant agents is an essential lever to protect living beings against pathogenic microorganisms and free radicals. In this regard, new functionalized pyrazoles have been synthesized using a simple and accessible approach. The synthesized aminobenzoylpyrazoles 3a-h and pyrazole-sulfonamides 4a-g were obtained in good yields and were evaluated in vitro for their antimicrobial and antioxidant activities. The structures of the synthesized compounds were determined using IR, NMR, and mass spectrometry. The structure of the compound 4b was further confirmed by single crystal X-ray diffraction. The results of the in vitro screening show that the synthesized pyrazoles 3 and 4 exhibit a promising antimicrobial and antioxidant activities. Among the tested compounds, pyrazoles 3a, 3f, 4e, 4f, and 4g have exhibited remarkable antimicrobial activity against some microorganisms. In addition, compounds 3a, 3c, 3e, 4a, 4d, 4f, and 4g have shown a significant antioxidant activity in comparison with the standard butylhydroxytoluene (BHT). Hence, compounds 3a, 4f, and 4g represent interesting dual acting antimicrobial and antioxidant agents. In fact, pyrazole derivatives bearing sulfonamide moiety (4a-g) have displayed an important antimicrobial activity compared to pyrazoles 3a-h, this finding could be attributed to the synergistic effect of the pyrazole and sulfonamide pharmacophores. Furthermore, Molecular docking results revealed a good interaction of the synthesized compounds with the target proteins and provided important information about their interaction modes with the target enzyme. The results of the POM bioinformatics investigations (Petra, Osiris, Molinspiration) show that the studied heterocycles present a very good non toxicity profile, an excellent bioavailability, and pharmacokinetics. Finally, an antiviral pharmacophore (O δ-, O δ-) was evaluated in the POM investigations and deserves all our attention to be tested against Covid-19 and its Omicron and Delta mutants.

Triazoles and Their Derivatives: Chemistry, Synthesis, and Therapeutic Applications.

Among the nitrogen-containing heterocyclic compounds, triazoles emerge with superior pharmacological applications. Structurally, there are two types of five-membered triazoles: 1,2,3-triazole and 1,2,4-triazole. Due to the structural characteristics, both 1,2,3- and 1,2,4-triazoles are able to accommodate a broad range of substituents (electrophiles and nucleophiles) around the core structures and pave the way for the construction of diverse novel bioactive molecules. Both the triazoles and their derivatives have significant biological properties including antimicrobial, antiviral, antitubercular, anticancer, anticonvulsant, analgesic, antioxidant, anti-inflammatory, and antidepressant activities. These are also important in organocatalysis, agrochemicals, and materials science. Thus, they have a broad range of therapeutic applications with ever-widening future scope across scientific disciplines. However, adverse events such as hepatotoxicity and hormonal problems lead to a careful revision of the azole family to obtain higher efficacy with minimum side effects. This review focuses on the structural features, synthesis, and notable therapeutic applications of triazoles and related compounds.

Optimized canopy structure improves maize grain yield and resource use efficiency

Improved canopy structure was instrumental in setting maize yield records, and yet it has rarely been examined in China. At Qitai Farm in Xinjiang, we conducted a 4‐year field experiment using China's six highest‐yielding maize hybrids sorted into three yield level groups that were grown at similar growth durations and at optimum densities. The average yield of high‐yield level (HL, 22.3 Mg ha−1) was 7.2% and 24.6% higher than that of medium‐yield level (ML) and low‐yield level (LL), respectively. For each yield level, we measured morphological traits that influence canopy structure and yield. They included plant height, ear height, ear ratio, internode length, leaf numbers, leaf angle, LOV, leaf area, and spatial density of leaf area. Among the preceding morphological traits of the three yield levels, HL’s best optimized the canopy structure, as shown by improved light distribution (19.0% light transmission at the ear) and increased light interception per unit leaf area per day (LIPA, 51.7 MJ m−2 day−1) in the canopy. In comparison, light transmission was 12.2% and 15.9% at the ear and the total LIPAs were 37.2 and 29.0 MJ m−2 day−1 at silking for ML and LL, respectively. HL had significantly longer leaf area duration and a higher photosynthetic rate, especially at the grain filling stage, and its total accumulated biomass at maturity was significantly better (by 13.9%) than LL’s. HL’s harvest index (0.54) was significantly higher than that of ML (0.52) and LL (0.48). HL’s radiation and heat use efficiencies were 2.61% and 1.37 g °C−1 day−1 m−2, both significantly greater than those of ML and LL. Therefore, optimum maize plant types can significantly improve canopy structure and increase resource use efficiency and grain yield.

Synthesis and Characterization of Dihydrouracil Analogs Utilizing Biginelli Hybrids

Dihydrouracil presents a crucial intermediate in the catabolism of uracil. The vital importance of uracil and its nucleoside, uridine, encourages scientists to synthesize novel dihydrouracils. In this paper, we present an innovative, fast, and effective method for the synthesis of dihydrouracils. Hence, under mild conditions, 3-chloroperbenzoic acid was used to cleave the carbon–sulfur bond of the Biginelli hybrids 5,6-dihydropyrimidin-4(3H)-ones. This approach led to thirteen novel dihydrouracils synthesized in moderate-to-high yields (32–99%).

An insight into the recent developments in anti-infective potential of indole and associated hybrids.

Prevention, accurate diagnosis, and effective treatment of infections are the main challenges in the overall management of infectious diseases. The best example is the ongoing SARs-COV-2(COVID-19) pandemic; the entire world is extremely worried about at present. Interestingly, heterocyclic moieties provide an ideal scaffold on which suitable pharmacophores can be designed to construct novel drugs. Indoles are amongst the most essential class of heteroaromatics in medicinal chemistry, which are ubiquitous across natural sources. The aforesaid derivatives have become invaluable scaffolds because of their wide spectrum therapeutic applications. Therefore, many researchers are focused on the design and synthesis of indole and associated hybrids of biological relevance. Hence, in the present review, we concisely discuss the indole containing natural sources, marketed drugs, clinical candidates, and their biological activities like antibacterial, antifungal, anti-TB, antiviral, antimalarial, and anti-leishmanial activities. The structure-activity relationships study of indole derivatives is also presented for a better understanding of the identified structures. The literature data presented for the anti-infective agents herein covers largely for the last twelve years.

Synthesis and in vitro Study of Artemisinin/Synthetic Peroxide-Based Hybrid Compounds against SARS-CoV-2 and Cancer.

The newly emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cause life-threatening diseases in millions of people worldwide, in particular, in patients with cancer, and there is an urgent need for antiviral agents against this infection. While in vitro activities of artemisinins against SARS-CoV-2 and cancer have recently been demonstrated, no study of artemisinin and/or synthetic peroxide-based hybrid compounds active against both cancer and SARS-CoV-2 has been reported yet. However, the hybrid drug's properties (e. g., activity and/or selectivity) can be improved compared to its parent compounds and effective new agents can be obtained by modification/hybridization of existing drugs or bioactive natural products. In this study, a series of new artesunic acid and synthetic peroxide based new hybrids were synthesized and analyzed in vitro for the first time for their inhibitory activity against SARS-CoV-2 and leukemia cell lines. Several artesunic acid-derived hybrids exerted a similar or stronger potency against K562 leukemia cells (81-83 % inhibition values) than the reference drug doxorubicin (78 % inhibition value) and they were also more efficient than their parent compounds artesunic acid (49.2 % inhibition value) and quinoline derivative (5.5 % inhibition value). Interestingly, the same artesunic acid-quinoline hybrids also show inhibitory activity against SARS-CoV-2 in vitro (EC50 13-19 µm) and no cytotoxic effects on Vero E6 cells (CC50 up to 110 µM). These results provide a valuable basis for design of further artemisinin-derived hybrids to treat both cancer and SARS-CoV-2 infections.

Available Technologies and Commercial Devices to Harvest Energy by Human Trampling in Smart Flooring Systems: A Review

Technological innovation has increased the global demand for electrical power and energy. Accordingly, energy harvesting has become a research area of primary interest for the scientific community and companies because it constitutes a sustainable way to collect energy from various sources. In particular, kinetic energy generated from human walking or vehicle movements on smart energy floors represents a promising research topic. This paper aims to analyze the state-of-art of smart energy harvesting floors to determine the best solution to feed a lighting system and charging columns. In particular, the fundamentals of the main harvesting mechanisms applicable in this field (i.e., piezoelectric, electromagnetic, triboelectric, and relative hybrids) are discussed. Moreover, an overview of scientific works related to energy harvesting floors is presented, focusing on the architectures of the developed tiles, the transduction mechanism, and the output performances. Finally, a survey of the commercial energy harvesting floors proposed by companies and startups is reported. From the carried-out analysis, we concluded that the piezoelectric transduction mechanism represents the optimal solution for designing smart energy floors, given their compactness, high efficiency, and absence of moving parts.